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91.
92.
Cold induction of EARLI1, a putative Arabidopsis lipid transfer protein, is light and calcium dependent 总被引:3,自引:0,他引:3
As sessile organisms, plants must adapt to their environment. One approach toward understanding this adaptation is to investigate environmental regulation of gene expression. Our focus is on the environmental regulation of EARLI1, which is activated by cold and long‐day photoperiods. Cold activation of EARLI1 in short‐day photoperiods is slow, requiring several hours at 4 °C to detect an increase in mRNA abundance. EARLI1 is not efficiently cold‐activated in etiolated seedlings, suggesting that photomorphogenesis is necessary for its cold activation. Cold activation of EARLI1 is inhibited in the presence of the calcium channel blocker lanthanum chloride or the calcium chelator EGTA. Addition of the calcium ionophore Bay K8644 results in cold‐independent activation of EARLI1. These data suggest that EARLI1 is not an immediate target of the cold response, and that calcium flux affects its expression. EARLI1 is a putative secreted protein and has motifs found in lipid transfer proteins. Over‐expression of EARLI1 in transgenic plants results in reduced electrolyte leakage during freezing damage, suggesting that EARLI1 may affect membrane or cell wall stability in response to low temperature stress. 相似文献
93.
《Animal : an international journal of animal bioscience》2018,12(2):205-214
Despite single nucleotide polymorphism (SNP) availability and frequent cost reduction has allowed genome-wide association studies even in complex traits as tick resistance, the use of this information source in SNP by environment interaction context is unknown for many economically important traits in cattle. We aimed at identifying putative genomic regions explaining differences in tick resistance in Hereford and Braford cattle under SNP by environment point of view as well as to identify candidate genes derived from outliers/significant markers. The environment was defined as contemporary group means of tick counts, since they seemed to be the most appropriate entities to describe the environmental gradient in beef cattle. A total of 4363 animals having tick counts (n=10 673) originated from 197 sires and 3966 dams were used. Genotypes were acquired on 3591 of these cattle. From top 1% SNPs (410) having the greatest effects in each environment, 75 were consistently relevant in all environments, which indicated SNP by environment interaction. The outliers/significant SNPs were mapped on chromosomes 1, 2, 5, 6, 7, 9, 11, 13, 14, 15, 16, 18, 21, 23, 24, 26 and 28, and potential candidate genes were detected across environments. The presence of SNP by environment interaction for tick resistance indicates that genetic expression of resistance depends upon tick burden. Markers with major portion of genetic variance explained across environments appeared to be close to genes with different direct or indirect functions related to immune system, inflammatory process and mechanisms of tissue destruction/repair, such as energy metabolism and cell differentiation. 相似文献
94.
Niels Abrahamsen Alberto Martinez Torben Kjær Leif Søndergaard Mary Bownes 《Molecular & general genetics : MGG》1993,237(1-2):41-48
Summary The three yolk protein genes (yp) of Drosophila melanogaster are transcribed in a sex- and tissue-limited fashion. We have searched for cis-regulatory sequences in regions flanking yp1 and yp2 to identify the elements that confer female-specific expression in the fat body. One such 127 by element has previously been identified in this region. We show here the existence of two additional regions which confer female fat body-specific expression on an Adh reporter gene and on the native yp2 gene, respectively. This suggests some redundancy in the regulation of expression of the yp genes. Computer searches for putative binding sites for the DSX protein, which regulates sex-specific expression of the yp genes, revealed several such sites in our constructs. However, the significance of these is unclear since many such sites also occur in genes which one would not expect to be regulated in a sex-specific manner (e.g. Adh, Actin 5C). We suggest that DSX acts in concert with other proteins to mediate sex- and tissue-specific expression of the yp genes. 相似文献
95.
96.
This study explores methods to use information gathered from genomics technology to understand evolutionary relationships
in the hyperdiverse legume group Neo-Astragalus. These species inhabit deserts and mountains of North and South America, and
even though the monophyly of the group is well established, relationships within it are still poorly understood. Plastid genes,
commonly used to infer phylogenies in plants, are usually not useful for closely related taxa because of low levels of genetic
variation. TheMedicago truncatula genome project provided a suite of candidate nuclear loci with high levels of variation that might prove suitable for low-level
phylogenetics. This paper reports the development of methods for screening a large number of these nuclear loci, and detailed
analysis of four of them. Four different patterns of phylogenetic diversification occur in the loci sampled from these genomes
ofAstragalus species. One locus (CNGC4) was single copy and could be directly used in phylogenetic analyses. Two loci (ARG10 and FENR)
showed patterns strongly suggestive of duplication events in some taxa, and one locus (tRALS) has apparently undergone a cryptic
duplication, making it very difficult to diagnose. Potential methods for using the information provided by these loci are
discussed. 相似文献
97.
An explosion set off in a limestone quarry located in the surroundings of Rapino (Chieti, central Italy), in the National Park of the Maiella Massif, exposed a small cave containing a Late Pleistocene fauna and Mousterian tools. Amongst the specimens is a fairly well preserved dorsal portion of a left mandibular corpus with associated M1 and M2 attributable to Macaca. The Grotta degli Orsi Volanti specimen is the very first macaque ever found in Abruzzo, but it also is one of the most recent, and the southern and easternmost known from Italy. 相似文献
98.
Xiang Zou 《World journal of microbiology & biotechnology》2005,21(6-7):1267-1271
Summary Effects of nutritional factors on exopolysaccharide production by submerged cultivation of the medicinal mushroom Oudemansiella radicata were investigated in shake flasks. Sucrose and peptone were optimal carbon and nitrogen sources for cell growth and exopolysaccharide
production. The exopolysaccharide production was increased with an increase in initial sucrose concentration within the range
of 10–40 g l−1 and initial peptone concentration within the range of 1–3 g l−1. To enhance further exopolysaccharide production, the effect of carbon/nitrogen ratios was studied using central composite
design (CCD) and response surface analysis. The maximum exopolysaccharide production of 2.67 ± 0.15 g l−1 was achieved in medium with optimized carbon and nitrogen sources, i.e. 39.3 g sucrose l−1 and 3.16 g peptone l−1 in the same cultivation conditions. The information obtained is helpful for the hyperproduction of exopolysaccharide by submerged
cultivation of O. radicata on a large scale. 相似文献
99.
Cell wall is a key structure of the plant organism engaged in numerous functions, and plants spend enormous resources on cell wall formation. Cell wall components are the most widespread organic substances on the Earth. However important is assembling plant cell wall polysaccharides, this process has been insufficiently studied by the methods of molecular genetics; in particular, too little is known of the genes that code for the relevant enzymes (glycosyltransferases, GT). The review addresses the current situation by expounding on GT classification, describing the characteristics of enzymes that synthesize cell wall polysaccharides, and summing up the existing knowledge of already identified and putative cellulose and callose synthases and GT localized in the Golgi apparatus. The methodology for searching and characterizing new genes that participate in cell wall formation is under discussion.__________Translated from Fiziologiya Rastenii, Vol. 52, No. 3, 2005, pp. 443–462.Original Russian Text Copyright © 2005 by Gorshkova, Nikolovski, Finaev. 相似文献
100.
Gene expression of the biosynthetic enzymes and biosynthesis of starch during Rice Grain development 总被引:1,自引:0,他引:1
Amylose and amylopectin are determinants of the physicochemical properties for starch and grain quality in rice. Their biosynthesis
is catalyzed by the interplay of ADP-glucose pyrophosphorylase (AGPase), granule-bound starch synthase (GBSS), soluble starch
synthase (SSS), a starch branching enzyme (SBE), and a starch debranching enzyme (SDE). In this study, the genes for these
enzymes were highly expressed 7 to 28 days after flowering during grain development, and their expression closely matched
increases in both starch content and grain weight Among all the tested cultivars, amylose contents in the rice grains remained
essentially constant throughout their development The AGPase gene was highly expressed in the high-yield cultivars of both
glutinous and non-glutinous rice. The SSS gene was actively expressed when mature GBSS mRNA decreased. Genes responsible for
amylopectin biosynthesis were simultaneously expressed in the late stage of grain development. We have now demonstrated that
the expression patterns of starch biosynthetic genes differ between glutinous and non-glutinous rice, and between Tongil (a
Japonica/ Indica hybrid) and Japonica types. 相似文献